Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Nutrition & Metabolism

Table 1 Composition and nutrient levels of the experimental vitamin D₃-deficient diets

Ingredients (%)	Composition	Calculated nutrient levels^c	Value
Corn (7.8% crude protein)	66.45	Metabolic energy(MJ /kg)	11.30
Soybean meal (43% crude protein)	22.80	Crude Protein(%)	15.52
Limestone	8.20	Calcicum(%)	3.60
calcium hydrophosphate	1.70	Available phosphorus(%)	0.39
Sodium chloride	0.30	Lysine(%)	0.75
DL-methionine (98%)	0.12	Methionine(%)	0.37
Choline chloride (50%)	0.10	Met+Cys(%)	0.68
Vitamin premix^a	0.03	Tryptophane(%)	0.18
Trace elements premix^b	0.30	Threonine(%)	0.57

^aProvided per kilogram of diet: vitamin A, 6000 IU; vitamin E, 21 IU; vitamin E, 21 IU; vitamin K3, 4.2 mg; vitamin B1, 3 mg; vitamin B2, 10.2 mg; folic acid, 0.9 mg; pantothenic acid, 15 mg; niacin, 45 mg; vitamin B6, 5.4 mg; vitamin B12, 24 μg; biotin, 0.15 mg. VD₃ added alone. Prepared with a small mixing machine after premix mixing. High performance liquid chromatography to detect the actual content, and the calculation of the value of the match before the feed preparation
^bMineral premix provided per kilogram of complete diet: copper, 6.8 mg; iron, 66 mg; zinc, 83 mg; manganese, 80 mg; iodine, 1 mg; Se 0.3 mg
^c Nutrient levels were calculated from data provided by Feed Database in China (2012)

ISSN: 1743-7075