Table 2 Sequences of primers for quantitative real-time PCR
Target gene | Primer sequence 5′ → 3′ | Product size (bp) | Annealing temperature (°C) | GenBank No. | Efficiency (%) |
|---|---|---|---|---|---|
VDR | F: TGGGAAAGGCGATGCTGATG | 169Â bp | 59.0 | NM_205098.1 | 86.5 |
R: GATGCGAGACATGCAGATG | Â | Â | Â | Â | |
RXR | F: GATGCGAGACATGCAGATG | 161Â bp | 55.0 | XM_015279790.1 | 96.3 |
R: CGGGGTATTTGTGCTTG | Â | Â | Â | Â | |
TLR2 | F: ACCTTCTGCACTCTGCCATT | 131Â bp | 58.5 | NM_204278.1 | 94.4 |
R: TGTGAATGAAGCACCGGTAA | Â | Â | Â | Â | |
TLR4 | F: CCACTATTCGGTTGGTGGAC | 86Â bp | 59.0 | NM_001030693.1 | 98.1 |
R: ACAGCTTCTCAGCAGGCAAT | Â | Â | Â | Â | |
NF-κB | F: ACCCCTTCAATGTGCCAATG | 274 bp | 59.3 | NM_205129.1 | 80.5 |
R: TCAGCCCAGAAACGAACCTC | Â | Â | Â | Â | |
TNF-α | F: CCCCTACCCTGTCCCACAA | 67 bp | 60.7 | NM204267.1 | 107.9 |
R: TGAGTACTGCGGAGGGTTCAT | Â | Â | Â | Â | |
IFN-γ | F: AAAGCCGCACATCAAACACA | 64 bp | 58.8 | NM_205149.1 | 90.4 |
R: GCCATCAGGAAGGTTGTTTTTC | Â | Â | Â | Â | |
IL-1β | F: CAGCAGCCTCAGCGAAGAG | 86 bp | 60.5 | NM_204524.1 | 98.7 |
R: CTGTGGTGTGCTCAGAATCCA | Â | Â | Â | Â | |
IL-6 | F: AGATGGTGATAAATCCTGATGA | 150Â bp | 54.5 | NM_204628.1 | 130.4 |
R: CGGTTTTCTCCATAAATGAAGT | Â | Â | Â | Â | |
IL-8 | F: GGCTTGCTAGGGGAAATGA | 200Â bp | 59.0 | NM_205498.1 | 88.6 |
R: AGCTGACTCTGACTAGGAAACTGT | Â | Â | Â | Â | |
LYZ | F: GACGATGTGAGCTGGCAG | 225Â bp | 57.1 | NM_205281.1 | 98.4 |
R: GGATGTTGCACAGGTTCC | Â | Â | Â | Â | |
iNOS | F: GAACAGCCAGCTCATCCGATA | 103Â bp | 59.8 | NM_204961.1 | 78.1 |
R: CCCAAGCTCAATGCACAACTT | Â | Â | Â | Â | |
pIgR | F: ATGAAGCAGAGCCAGGAGAC | 103Â bp | 59.7 | NM_001044644.1 | 106.4 |
R: GAGTAGGCGAGGTCAGCATC | Â | Â | Â | Â | |
β-actin | F: GAGAAATTGTGCGTGACATCA | 282 bp | 58.0 | NM 205518 | 97.8 |
R: CCTGAACCTCTCATTGCCA | Â | Â | Â | Â |