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Table 6 Effect of vitamin D3 on serum MPO, complement and natural antibody of laying hens infected with LPS*

From: Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Items

Dietary VD3 levels, IU/kg

 

P-values

0

3000

Saline

LPS

Saline

LPS

SEM

VD3

LPS

Interaction

MPO (ng/ml)

39.23a

21.04b

24.29b

22.62b

1.98

0.011

<  0.001

0.003

C3 (g/L)

0.87

0.78

0.88

0.76

0.03

0.896

0.054

0.751

C4 (g/L)

0.37

0.23

0.25

0.20

0.03

0.141

0.058

0.367

C5 (μg/ mL)

73.94

55.7

79.06

52.69

4.47

0.896

0.012

0.615

IgG (mg/mL)

21.4

17.96

18.76

15.25

1.17

0.038

0.009

0.977

IgM (μg/mL)

253.01b

252.67b

328.70a

163.04c

35.63

0.851

0.041

0.043

  1. *Data are presented as mean ± SEM (n = 6 hens /group)
  2. a-cData in the same row with no common superscript differ significantly (P <  0.05)
  3. SEM standard error of the mean; LPS lipopolysaccharide; MPO myeloperoxidase; C3 complement 3; C4 complement 4; C5 complement 5; IgG immunoglobulins G; IgM immunoglobulins M