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Table 9 Gene expression in the spleens of laying hens fed diets with or without supplemental dietary VD3*

From: Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Items

Dietary VD3 levels, IU/kg

 

P-values

0 IU/kg

3000 IU/kg

Saline

LPS

Saline

LPS

SEM

VD3

LPS

Interaction

VDR

1.52

1.22

1.16

1.71

0.14

0.998

0.629

0.200

RXR-α

1.96

1.78

1.14

1.05

0.14

0.020

0.824

0.344

TLR2

1.82a

0.76b

0.98b

1.04b

0.14

0.143

0.094

0.042

TLR4

1.13

3.17

1.22

1.97

0.24

0.300

0.020

0.620

NF-κB

1.56b

2.64a

1.08b

1.58b

0.18

0.009

0.011

0.029

TNF-α

0.98

1.25

1.12

1.74

0.11

0.130

0.061

0.815

IFN-γ

0.73

0.89

0.98

0.98

0.11

0.559

0.053

0.443

IL-1β

1.08

0.88

1.28

0.86

0.19

0.700

0.220

0.960

IL-6

0.51

2.30

0.81

1.94

0.23

0.893

< 0.001

0.439

IL-8

1.58

1.80

1.11

1.97

0.19

0.879

0.940

0.181

LYZ

1.84a

0.52b

0.47b

1.43a

0.20

0.561

0.453

0.003

iNOS

2.10

1.82

1.30

1.12

0.17

0.016

0.750

0.258

pIgR

3.75a

1.42b

0.96b

2.08a

0.33

0.048

0.241

0.003

  1. *Data are presented as mean ± SEM (n = 6 hens /group)
  2. a-c Mean values with different letters differ significantly (p < 0.05)
  3. SEM standard error of the mean; LPS lipopolysaccharide; VDR vitamin D receptor; RXR retinoid X receptor; TLR Toll-like receptor; NF-κB Nuclear factor kB; TNF-α tumor necrosis factor; IFN interferon; IL interleukin; LYZ lysozyme; iNOS inducible nitric oxide synthase; pIgR polyglobulin receptor