Fig. 1

Schematic representataion of protocol used for BCAAs supplementation and exercise. As depicted, acclimation was performed for two weeks. Then, mice were randomly divided to training (n = 6) and sedantry (Sed; n = 6) groups. Training groups were EX as exercised group. 20BCAA and 60BCAA groups were mice supplemented with 20 and 60 mg/mL of BCAAs respectively. 20BCAA/EX and 60BCAA/EX were exercised groups supplemented with 20 and 60 mg/mL of BCAAs respectively. BCAAs supplementaion was performed by oral gavage (500 μL) 5 days per week. On the other hand mice were excercised by placing on the motor-driven treadmill as described in materials and methods for 5 days per week. Exercise and BCAAs supplemention was carried out as an intervention for 8 weeks. One day after the final session of intervention (gavage or exercise), mice were sacrified for further experiments