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Fig. 12 | Nutrition & Metabolism

Fig. 12

From: PPARγ/Pgc-1α-Fndc5 pathway up-regulation in gastrocnemius and heart muscle of exercised, branched chain amino acid diet fed mice

Fig. 12

Modulation in relative expression level of Sirt1 in gastrocnemius and heart muscles of exercised and BCAAs supplemented mice. qRT-PCR on cDNA samples of gastrocnemius muscle (a) and heart (b) tissues derived from exercised and 20 mg/mL BCAAs supplemented mice (20BCAA/Ex) indicated a significant increase of Sirt1 transcript level (p < 0.05). Data are shown as mean ± SEM (Standard Error of Mean) for each group (n = 6, ). + indicates statistically significant difference with Sedentary group at p < 0.05, * indicates statistically significant difference with 20BCAA/Ex group at p < 0.05, # indicates statistically significant difference with 60BCAA/Ex group at p < 0.05, & indicates statistically significant difference with Ex group at p < 0.05, $ indicates statistically significant difference with 20BCAA group at p < 0.05, ^ indicates statistically significant difference with 60BCAA group at p < 0.05. Data of qRT-PCR were obtained on triplicate data sets for each sample and analyzed by two-way analysis of variance (ANOVA) and tukey’s post hoc test

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