Protective effects of γ-aminobutyric acid against H 2 O 2 -induced oxidative stress in RIN-m5F pancreatic cells

Nutrition & Metabolism

Table 1 GABA causes an increment in the antioxidant status of RINm5F exposed to H₂O₂^*

	T-AOC	CAT	SOD	GSH-Px	MDA
	(U/mg prot)	(U/mg prot)	(U/mg prot)	(U/mg prot)	(nM/mg prot)
Control	1.20 ± 0.15^d	8.65 ± 0.63^d	16.31 ± 1.21^d	138.91 ± 11.02^d	3.18 ± 0.26^e
H₂O₂	0.26 ± 0.06^a	2.30 ± 0.19^a	4.49 ± 0.45^a	57.31 ± 5.97^a	6.50 ± 0.52^a
H₂O₂ + 50 μmol/L GABA	0.40 ± 0.05^a	2.57 ± 0.45^ab	5.57 ± 0.48^a	63.39 ± 4.02^a	6.17 ± 0.54^ab
H₂O₂ + 100 μmol/L GABA	0.59 ± 0.07^b	3.19 ± 0.22^b	8.29 ± 0.57^b	75.80 ± 8.31^b	5.52 ± 0.29^bc
H₂O₂ + 200 μmol/L GABA	0.76 ± 0.07^c	4.71 ± 0.38^c	11.71 ± 0.74^c	91.00 ± 10.07^c	5.02 ± 0.29^cd

Control, control medium; H₂O₂, medium containing 100 μmol/L H₂O₂; H₂O₂ + (50 μmol/L, 100 μmol/L, 200 μmol/L) GABA, medium containing 100 μmol/L H₂O₂ and GABA; T-AOC Total antioxidant capacity, CAT Catalase, SOD, Superoxide dismutase, GSH-PX Glutathione peroxidase, MDA Malondialdehyde
*Values are expressed as mean ± SD (n = 8) of three separate experiments. Means with different superscript letters within a same column gare significantly different (P < 0.05)