Fig. 2

Representative gross anatomy, histology and ultrasound images from mice diagnosed with steatosis, steatohepatitis and steatohepatitis with HCC. The gross anatomy showed livers with a light-yellow color in the mice with simple steatosis, steatohepatitis and steatohepatitis with HCC. The HCC nodules were found in the mice treated with HFD + DEN. Histological changes indicated by H&E staining confirmed the simple steatosis as widely distributed lipid drops in hepatic parenchyma, steatohepatitis as inflammatory infiltration and hepatocyte ballooning, and HCC as destruction of normal hepatic architecture, plate-like growth and abnormal cytological structure of hepatocytes. The ultrasound images show increased intensity of echogenicity due to fatty infiltration in simple steatosis and steatohepatitis. Hyperechoic masses were detected in the ultrasound images from steatohepatitic mice with HCC. Scale bar size in HE staining = 200 μM