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Table 1 Fold change in the relative abundance of Tnnt3 splice forms

From: Palmitate- and C6 ceramide-induced Tnnt3 pre-mRNA alternative splicing occurs in a PP2A dependent manner

Tnnt3 splice form size (bp)

BSA

PA

OA

LA

710

1.00 a

1.228 ± 0.240 a

1.042 ± 0.099 a

1.091 ± 0.067 a

725

1.00 a, c

1.212 ± 0.109 a

0.970 ± 0.041 b, c

1.022 ± 0.078 a, c

728

1.00 a

0.713 ± 0.098 a

0.832 ± 0.239 a

0.875 ± 0.270 a

737

1.00 a

1.851 ± 0.143 b

0.943 ± 0.053 a

0.921 ± 0.180 a

739

1.00 a

1.438 ± 0.202 a

1.327 ± 0.350 a

1.115 ± 0.403 a

742

1.00 a

1.026 ± 0.057 a

0.946 ± 0.044 a

0.978 ± 0.117 a

751

1.00 a

1.198 ± 0.209 a

0.662 ± 0.247 a

0.794 ± 0.063 a

754

1.00 a, b

1.233 ± 0.177 a

0.764 ± 0.150 b

0.826 ± 0.069 b

757

1.00 a

1.105 ± 0.061 a

0.923 ± 0.091 a

1.013 ± 0.086 a

763

1.00 a

0.989 ± 0.104 a

0.860 ± 0.097 a

0.884 ± 0.069 a

769

1.00 a

1.071 ± 0.036 a

0.906 ± 0.124 a

0.804 ± 0.112 a

775

1.00 a

1.334 ± 0.177 a

0.990 ± 0.233 a

0.977 ± 0.254 a

778

1.00 a

0.999 ± 0.164 a

1.151 ± 0.017 a

1.085 ± 0.119 a

781

1.00 a

0.950 ± 0.030 a

1.104 ± 0.014 b

1.114 ± 0.033 b

790

1.00 a

0.839 ± 0.070 a

0.833 ± 0.042 a

0.790 ± 0.157 a

793

1.00 a

0.837 ± 0.036 b, c

0.935 ± 0.054 a, c

0.776 ± 0.078 b

795

1.00 a, b

0.953 ± 0.044 a

1.071 ± 0.043 b

1.216 ± 0.078 c

807

1.00 a

0.966 ± 0.030 a, b

0.955 ± 0.035 a, b

0.909 ± 0.020 b

  1. L6 myotubes were treated for 24 h with 150 μM palmitate, oleate, or linoleate (PA, OA, LA, respectively) conjugated to BSA or an equal volume of BSA alone. The fold change in the relative abundance of Tnnt3 splice forms was assessed by capillary electrophoresis. Data are presented as means ± SEM from three independent experiments using three replicates per treatment. Statistical significance was assessed by One-way ANOVA and Fishers LSD post-hoc test for multiple comparisons. Statistically different means are denoted with different letters (p ≤ 0.05)