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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Involvement of PPARγ/FSP27 in the pathogenic mechanism underlying insulin resistance: tipping the balance between lipogenesis and fat storage in adult catch-up growth rats

Fig. 3

Lipogenesis and fat storage capacity in VAT during CUGA. a FAS activity and expression of FAS mRNA (b) and protein (c). d 2-DG uptake in respond to administration of insulin in hyperinsulinemic–euglycemic clamp. e Relative amounts of LPL mRNA . Analysis of PPARγ (f) and FSP27 (h) gene expression. Analysis of PPARγ (g) and FSP27 (i) protein expression. jThe relative protein levels of PPARγ (black triangle) and FSP27 (white triangle) standardized by controls. k Gene expression of perilipin1. l Protein expression of perilipin1. m Representative immunoblot analysis for FAS, PPARγ, FSP27 and Perilipin1. β-actin served as a loading control. All experiments consisted of n = 4/group. White bars, AL groups; black bars, RN groups. *P < 0.05, **P < 0.01 versus the corresponding AL groups

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Nutrition & Metabolism

ISSN: 1743-7075

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