Fig. 2

RSV attenuates PA-induced oxidative stress in HUVECs through TyrRS-PARP1 pathway. Cells were treated as indicated and labeled by DCFH-DA probe. a-b: Representative images (a) and quantification of intracellular ROS levels by FCM assay (b) c: Quantification of ROS levels by the microplate reader. d-f: Quantification of MDA of the medium (d) and cell lysates (e), the activity of SOD of the cell lysates (f). g: Cells were pretreated with siRNA of TyrRS, PAPR1, and the vehicle and were treated as indicated. The fluorescence intensity of cells labeled by DCFH-DA was measured by the microplate reader. Values are expressed as means ± SD (n = 3); ^*p < 0.05, ^**p < 0.01 vs. the vehicle-treated control group; ^#p < 0.05, ^##p < 0.01 vs. vehicle + PA-treated group; ^$p < 0.05, ^$$p < 0.01 vs. vehicle + PA + RSV-treated group