Fig. 3

RSV maintains mitochondria function in PA-treated HUVECs through TyrRS-PARP1 pathway. Cells were treated with 200 μM PA for 16 h, co-cultured with 10 μM RSV for another 8 h and then labeled by JC-1 probe as described in the methods and materials section. a-b: Representative images (a) and quantification (b) of FCM on detecting red to green ratio of fluorescence intensity. c: Quantification of green to red ratio of fluorescence intensity by the microplate reader. d: Cells were pretreated with siRNA of the vehicle, TyrRS or PAPR1 for 24 h before cultured with 200 μM PA for 16 h and co-cultured with 10 μM RSV for another 8 h. Cells were then labeled by JC-1 probe. Quantification of green to red ratio of fluorescence intensity by microplate reader is displayed in the bar graph. Values are expressed as means ± SD (n = 3); ^*p < 0.05, ^**p < 0.01 vs. the vehicle-treated control group; ^#p < 0.05, ^##p < 0.01 vs. vehicle + PA-treated group; ^$p < 0.05, ^$$p < 0.01 vs. vehicle+PA + RSV-treated group