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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Resveratrol attenuates oxidative injury in human umbilical vein endothelial cells through regulating mitochondrial fusion via TyrRS-PARP1 pathway

Fig. 3

RSV maintains mitochondria function in PA-treated HUVECs through TyrRS-PARP1 pathway. Cells were treated with 200 μM PA for 16 h, co-cultured with 10 μM RSV for another 8 h and then labeled by JC-1 probe as described in the methods and materials section. a-b: Representative images (a) and quantification (b) of FCM on detecting red to green ratio of fluorescence intensity. c: Quantification of green to red ratio of fluorescence intensity by the microplate reader. d: Cells were pretreated with siRNA of the vehicle, TyrRS or PAPR1 for 24 h before cultured with 200 μM PA for 16 h and co-cultured with 10 μM RSV for another 8 h. Cells were then labeled by JC-1 probe. Quantification of green to red ratio of fluorescence intensity by microplate reader is displayed in the bar graph. Values are expressed as means ± SD (n = 3); *p < 0.05, **p < 0.01 vs. the vehicle-treated control group; #p < 0.05, ##p < 0.01 vs. vehicle + PA-treated group; $p < 0.05, $$p < 0.01 vs. vehicle+PA + RSV-treated group

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