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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: Resveratrol attenuates oxidative injury in human umbilical vein endothelial cells through regulating mitochondrial fusion via TyrRS-PARP1 pathway

Fig. 5

RSV regulates mitochondria fusion proteins through the TyrRS-PARP1 pathway. a-b: Cells were treated with 200 μM PA for 16 h, co-cultured with 10 μM RSV for another 8 h, and then proteins were extracted for western blot. The protein levels of OPA, MFN1, and MFN2 were analyzed by western blotting (a) and quantification displayed by bar graph (b). c-d: Cells were pretreated with siRNA of TyrRS (c-d) or PAPR1 (e-f) for 24 h before cultured with 200 μM PA for 16 h and co-cultured with 10 μM RSV for another 8 h. The protein levels of TyrRS, PARP1, OPA1, MFN1, and MFN2 were analyzed by western blotting (c, e) and quantification displayed by bar graph (d, f). Values are expressed as means ± SD (n = 3); *p < 0.05, **p < 0.01 vs. the vehicle-treated control group; #p < 0.05, ##p < 0.01 vs. vehicle + PA-treated group; $p < 0.05, $$p < 0.01 vs. vehicle + PA + RSV-treated group. A.U., arbitrary units

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