Fig. 2

Lipidomic analysis of HFD-induced myocardial changes in Ad-KO mice and the effect of adiponectin replenishment. a Lipidomic analyses were performed on heart tissues collected from Ad-KO mice treated as described in Fig. 3 and data are shown for the myocardial content of different species of diacylglycerols, sphingomyelins, and ceramides. Left panel: influence of HFD versus chow is shown as fold change observed in HF group versus chow-fed mice. Right panel: graph shows fold change in adiponectin-treated versus saline-treated within HFD group. Changes indicate a corrective effect of adiponectin. Data represent mean ± SEM; * significant difference between HF diet group and chow diet group; # significant difference between Ad and saline treatment within HF diet group; *,#, P < 0.05; **,##, P < 0.01; ***,###, P < 0.001, n = 5–6. The concentration of S1P in b) Heart (intracellular S1P) and c) Serum (extracellular S1P) in Ad-KO animals fed either a chow, 60% HFD or a 60% HFD plus Ad at a dosage of 3 μg/g for two weeks adiponectin using an ELISA kit. Data represent mean ± SEM, *P < 0.05 vs WT, ^#P < 0.05 vs HF, n = 3–5. d The concentration of S1P in H9c2 cells treated (intracellular S1P) and e) the media of these cells (extracellular S1P) with either 0.1 mM PA, with and without 35 μM AdipoRon (AR). *P < 0.05 vs BSA, ^#P < 0.05 vs PA, n = 4