Fig. 4

Reducing PA-induced ROS production with MnTBAP or AdipoRon attenuates cell death in H9c2 cells. Cell death in H9c2 cells measured using a a) LDH, b) MTT or c) Trypan blue assay. Following a preincubation with 0.5% FBS, cells were incubated with 0.1 mM FA for 1, 2, or 4 h or were also treated with the addition of 35 μM AdipoRon (AR) as indicated. For all graphs the y-axis is displayed as % toxicity relative to BSA. Data represent mean ± SEM; *P < 0.05 vs BSA control group, ^#P < 0.05 vs PA control group, n = 6. In another subset of cells 100 μM MnTBAP was added for 24 h following which d) LDH, e) MTT or f) Trypan blue was measured. For all graphs the y-axis is displayed as % toxicity relative to BSA. Data represent mean ± SEM; *P < 0.05 vs PA, ^#P < 0.05 vs PA + AR, n = 3