Fig. 5

Manipulation of S1P action significantly alters cell death in H9c2 cells. Cell death in H9c2 cells measured using a a) LDH, b) MTT or c) Trypan blue assay. Following a preincubation with 0.5% FBS, cells were incubated with 0.1 mM FA for 24 h and were also treated with the addition of 35 μM AdipoRon (AR), 5 μM SKI-II and 5 μM THI as indicated. For all graphs the y-axis is displayed as % toxicity relative to BSA. Data represent mean ± SEM; *P < 0.05 vs PA, ^#P < 0.05 vs PA + AR, ^%P < 0.05 vs PA + SKI-II, ^$P < 0.05 vs PA + THI, n = 3. In another subset of cells 2.5 μM S1P added for 24 h following which d) LDH, e) MTT or f) Trypan blue was measured. For all graphs the y-axis is displayed as % toxicity relative to BSA. Data represent mean ± SEM; P < 0.05 vs PA, ^#P < 0.05 vs PA + AR, n = 3