Fig. 3
From: A high-fat diet induces rapid changes in the mouse hypothalamic proteome
a Representative immunoblots showing changes in DRP-2 and beta-actin protein expression in response to fatty acid free BSA, PA and OA challenge in N42 cells b Quantification of DRP-2 bands normalised to beta-actin (n = 4 plates) BSA - bovine serum albumin, DRP-2 - dihydropyrimidinase-related protein 2, PA - palmitic acid, OA - oleic acid (* P < 0.05). c-e Representative fluorescence microscopy images of N42 hypothalamic neuronal mitochondria using MitoTracker® Red CMXRos. The red colour corresponds to mitochondria. Cells were challenged with, c fatty acid free BSA, d 200 μM PA and e 200 μM OA (Bar = 10 μm. Magnification = X100). f The percentage (%) area of the cell occupied by mitochondria after challenge. BSA - bovine serum albumin, PA - palmitic acid, OA - oleic acid (*P < 0.05, **P < 0.01, ***P < 0.001)