Fig. 4From: Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499The effects of myricetin on muscle fiber-type composition in L6 myotubes. Cell viability (a) was measured using CCK-8 assay. The mRNA expressions of slow-twitch myosin Myh7 (b), fast-twitch myosin Myh1 (c), Myh4 (d), and slow-twitch fiber biomarker myoglobin (e) were determined by real-time PCR. Protein levels of slow-twitch myosin Myh7 and slow-twitch fiber biomarkers (f) in L6 myotubes were measured by immunoblotting. Data are presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001 compared with controlBack to article page