Table 1 Primers used for Real-time PCR
From: Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499
Gene | Primer sequence | Ta(°C) | |
|---|---|---|---|
Myh1 | Forward | GCGACAGACACCTCCTTCAAGAAC | 60 |
Reverse | CCAGCCAGCCAGCGATGTTG | ||
Myh4 | Forward | CCATCACTGACGCCGCCATG | 60 |
Reverse | GTTCTTCTTCATCCGCTCCAGGTG | ||
Myh7 | Forward | GAGCAGGAGCTGATCGAGAC | 60 |
Reverse | ATGGCCTTCTTGGCCTTCTC | ||
Myh7b | Forward | CAACCTGGCTAAGTACCGCA | 60 |
Reverse | AATAAGGACAGGACAGCGGC | ||
Myoglobin | Forward | CACCATGGGGCTCAGTGATG | 60 |
Reverse | CTCAGCCCTGGAAGCCTAGC | ||
PGC-1α | Forward | GCAGCGGTCTTAGCACTCA | 60 |
Reverse | GACTGCGGTTGTGTATGGGA | ||
Sox6 | Forward | TCCATGGCTTTGTCACTTTCA | 60 |
Reverse | ATACACGAGGATCACCCCAG | ||
GAPDH | Forward | ACGGGAAGCTCACTGGCATGG | 60 |
Reverse | CGCCTGCTTCACCACCTTCTT | ||