Fig. 1

Construction and functional analysis of ApoA-IV knockout rats. a Deletion of 8 bp within the Apoa4 exon 2 using TALEN approach causes a frameshift, resulting in gene knockout. Western blot analysis confirmed deletion of ApoA-IV in the duodenum, liver and plasma. b Hepatic mRNA levels of Apoa4 and Apoc3 were detected. n = 6 WT, 4 KO. c Body weight of WT or KO rats when maintained on a normal rodent diet. n = 13 WT, 15 KO. d Food intake. n = 7 WT, 9 KO. e Blood glucose (n = 10), serum insulin (n = 4) and plasma glucagon (n = 4) levels were measured under random fed and 16-h fasted states. f Serum triglyceride (TG), cholesterol (Chol) and free fatty acid (FFA) were measured under random fed and 16-h fasted states. n = 6. g IPGTT (2 g/kg body weight). n = 6. h IPITT (1 U/kg body weight). n = 6. i Serum insulin levels after glucose injection. n = 4 WT, 5 KO. j IPPTT (1.5 g/kg body weight). n = 4 WT, 5 KO. **p < 0.01, ***p < 0.001 for KO versus WT, n.s., not significant. Error bars indicate S.E.M.