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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Resveratrol attenuates endothelial oxidative injury by inducing autophagy via the activation of transcription factor EB

Fig. 1

PA induces oxidative stress in HUVECs. a Cells were incubated with different concentrations (100, 150, 200, 250 and 300 μM) of PA. Cell viability was measured by CCK-8 detection kit as described in the Materials and methods section. b Cells were incubated with different time (12, 16, 20 and 24 h) of PA. Cell viability was measured by CCK-8 assay. c-e Intracellular ROS levels were estimated using a probe DCFH-DA by confocal microscopy (c) and The bar charts show quantification of the ROS generation levels expressed as the fold change (d), and by microplate reader, fluorescence was read at 485 nm for excitation and 520 nm for emission (e). f The levels of MDA were detected using an MDA detection kit. Values are presented as means ± SD (n = 6); *p < 0.05, **p < 0.01 compared with control groups

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