Fig. 3

Analysis of brown adipose tissue (BAT)-related gene and protein expression levels. a BAT-related gene expression levels in each test group. Test mice include those fed a high-fat diet as described in the “Materials and methods” section. Gene expression was analyzed by real-time PCR. Data are normalized against Gapdh and expressed as fold changes relative to the expression level in WT mice. b Detection of Ucp1 by a western blot analysis in BAT. Western blot analyses were performed as described in the “Materials and methods” section. c Ucp1 staining in BAT, bar: 100 μm. Brown parts are positively stained for Ucp1. *p < 0.05, **p < 0.01 ***p < 0.001 by Student’s t-test