Fig. 4

Analysis of white adipose tissue (WAT)-related gene and protein expression levels. a, b eWAT gene expression levels in each test group. Test mice include those fed a high-fat diet as described in the “Materials and methods” section. Gene expression was analyzed by real-time PCR. Data are normalized against Gapdh and expressed as fold changes relative to the expression level in WT mice. c Detection of Ucp1 by a western blot analysis in eWAT. Western blot analyses were performed as described in the “Materials and methods” section. Brown parts are positively stained for Ucp1. d Ucp1 staining, bar: 100 μm. *p < 0.05, **p < 0.01 ***p < 0.001 by Student’s t-test