Fig. 3

Overexpression of PLIN5 improved GSIS in long-term PA treated INS-1 cells. a GSIS of untransduced INS-1 cells and those transduced with Ad-GFP or Ad-PLIN5. b GSIS with or without 0.5 mM PA for 12 h in Ad-PLIN5 INS-1 cells compared with Ad-GFP control. c GSIS with or without 0.5 mM PA for 48 h in Ad-PLIN5 INS-1 cells compared with Ad-GFP control. Data expressed as the fold change taking GSIS of untransduced INS-1 cells (A) or Ad-GFP at 2.5 mM glucose (B and C) as 100%. d Q-PCR analysis of the mRNA levels of Cyclin D1 and Cyclin D2 in the INS-1 cells transduced with Ad-GFP or Ad-PLIN5. e Immunoblotting analysis of the levels of GLP-1 receptor (GLP-1R) in the INS-1 cells transduced with Ad-GFP or Ad-PLIN5. Values are means ± SE from three independent experiments, each conducted in triplicate. **P < 0.01, vs. indicated group