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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: Perilipin5 protects against lipotoxicity and alleviates endoplasmic reticulum stress in pancreatic β-cells

Fig. 4

Upregulation of PLIN5 in β-cells improves glucose tolerance without interfering with insulin sensitivity in HFD mice. a PLIN5 overexpression was confirmed in β-cells of mice (n = 5 for each group). Western blots showed that most of the AAV were delivered to β-cells (left) with a few also entering liver, muscle, and visceral fat (right) in mice. b Fasting and fed glucose levels of mice (n = 8 for each group). After HFD treatment for 5 months, AAV-PLIN5 and AAV-MIP were delivered to HFD mice and Chow mice was injected with saline by tail vein. One week after injection, fasting (12 h) and fed glucose (2 h) levels were determined from tail vein blood. c GTT of CHOW, HFD-AAV-MIP, and HFD-AAV-PLIN5 mice (n = 5–8 for each group). Ten to fourteen days after tail vein injection, GTT was conducted. Serum insulin at 0, 15, and 30 min after GTT were measured in different groups. d ITT of CHOW, HFD-AAV-MIP, and HFD-AAV-PLIN5 mice. AUC of ITT was calculated (n = 8 for each group). Values are means ± SE. AUCg: area under the curve of the GTT. GTT: glucose tolerance test. ITT: insulin tolerance test. *P < 0.05, **P < 0.01, vs. CHOW; # P < 0.05, ## P < 0.01, vs. HFD-AAV-MIP

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