Fig. 5

Upregulation of PLIN5 alleviated PA-induced cytotoxicity and apoptosis in INS-1 cells. INS-1 cells transfected with Ad-GFP or Ad-PLIN5 were incubated with control medium or 0.5 mM PA for 24 h. a Protective effect of PLIN5 on PA-induced impairment in cell viability. Cell viability was determined using CCK-8 assay. b Overexpression of PLIN5 protected against PA-induced apoptosis. Cell apoptosis was measured by flow cytometry (left). Apoptosis associated molecules expressions were determined by Western blots (right). Data were expressed as the fold change taking Ad-GFP as 100%. Values are means ± SE from three independent experiments, each conducted in triplicate. **P < 0.01, vs. Ad-GFP; ##P < 0.01 vs. Ad-GFP + PA