Fig. 6

Upregulation of PLIN5 attenuated PA-induced ER stress partially by enhancing FAO in INS-1 cells. a Expression of BiP and CHOP was examined by Western blot (left) and Q-PCR (right). INS-1 β-cells transduced by Ad-GFP or Ad-PLIN5 were treated with 0.5 mM PA alone or preincubation with etomoxir (ET) for 30 min and then incubated with 0.5 mM PA for 24 h. *P < 0.05, **P < 0.01, vs. Ad-GFP; #P < 0.05, ##P < 0.01 vs. Ad-GFP + PA. b Expression of CPT-1 and ACO was examined by Western blot. INS-1 β-cells were transduced by Ad-GFP or Ad-PLIN5 and then treated with control medium or 0.5 mM PA for 24 h. c Expression of BiP and CHOP was examined by Western blot (right). PLIN5 knockout induced by si-PLIN5 transfection was confirmed by Western blot (left). INS-1 β-cells co-transduced by different plasmids (CPT-1A overexpression plasmid and the control vector) and/or siRNA (PLIN5 siRNA and the control siRNA) were treated with 0.5 mM PA for 24 h. d Protective effect of CPT-1A overexpression on cell viability in PA treated INS-1 cells with PLIN5 deletion. Cell viability was determined using CCK-8 assay. **P < 0.01, vs. si-Con. e Protective effect of CPT-1A overexpression on PLIN5-induced improvement in cell viability. Cell viability was determined using CCK-8 assay. *P < 0.05, **P < 0.01, vs. si-Con. Values are means ± SE from three independent experiments, each conducted in triplicate