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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Dietary curcumin enhances insulin clearance in diet-induced obese mice via regulation of hepatic PI3K-AKT axis and IDE, and preservation of islet integrity

Fig. 3

CUR administration reduces lipid accumulation and enhances downstream insulin signaling in the liver of HFS mice. Control, HFS and HFS + CUR fed mice were fasted overnight after 16 weeks of treatment. a Liver sections from Control, HFS and HFS + CUR fed mice were stained with Oil Red O and imaged at 20x objective. Quantitative analysis was based on at least 140 images/group (n = 8 mice per group). b-f Protein extracts from liver homogenates were immunoblotted and detected by autoradiographical signals. The bands were subjected to densitometric quantifications. b phospho-AKTSer473 and total AKT analysis c PHLPP1 and β-tubulin d phosphor-PP2A A/B subunits and total PP2A A and B subunits e p85, p110α, β, or δ catalytic subunit, p110 class III and β-tubulin (n = 4 mice per group). f p110γ, p101, Gβ and GAPDH (n = 4 mice per group). Integrated density was quantified using ImageJ software. Values were normalized to total AKT, PP2A A/B subunits, β-tubulin or GAPDH, respectively. (*p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001 compared to Control; #p ≤ 0.05, ##p ≤ 0.01, ####p ≤ 0.0001 compared to HFS + CUR)

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