Fig. 6
From: Early and reversible changes to the hippocampal proteome in mice on a high-fat diet
Representative images of microtubule-associated protein 2 (MAP2) immunostaining captured with fluorescence microscopy showing dendritic morphology in hippocampal neurons challenged for 6 h with: A. BSA alone (control) B. 200 μM PA C. 200 μM DHA D. 200 μM PA plus 125 μM DHA E. 200 μM PA for 3 h followed by BSA for 3 h. Scale bar: 20 μm. Ai-Ei. Higher magnification fluorescence microscopy of MAP2 immunostaining showing dendritic morphology in hippocampal neurons challenged for 6 h with: Ai. BSA alone (control) Bi. 200 μM PA Ci. 200 μM DHA Di. 200 μM PA plus 125 μM DHA. Ei. 200 μM PA for 3 h followed by BSA for 3 h. Bovine serum albumin (BSA), palmitic acid (PA) and docosahexaenoic acid (DHA). Scale bar: 10 μm. Bovine serum albumin (BSA), palmitic acid (PA) and docosahexaenoic acid (DHA)