Fig. 5

ALP staining in primary OBs. (a) OBs were grown for 5 days in the indicated medium (con group contained 0.1% ethanol, ALA 1 μM, ALA 10 μM, ALA 100 μM). (b) OBs were grown for 5 days in the indicated medium (con group contained 0.1% ethanol and 2.4% BSA, PA 0.2 mM, PA 0.2 mM + ALA 1 μM, PA 0.2 mM + ALA 10 μM, PA 0.2 mM + ALA 100 μM). (c-d) ALP activity quantification was measured after different treatments. Data are shown as the mean ± SD (n = 3). ^**p < 0.01 versus vehicle treatment (con). ## p < 0.01 # p < 0.05 versus (PA 0.2 mM) treatment by one-way ANOVA