Fig. 3

Effect of a high-fat diet and moderate physical exercise on the Th17 (a) and Treg (b) fractions of total CD4+ lymphocytes in lymph nodes of tumor-bearing mice. C57BL6 mice were maintained on a diet containing either 60% or 10% of energy from lipids and were either trained on a treadmill at moderate intensity for 8 weeks or left untrained. Subsequently, B16F10 tumor cells were injected, and animals were evaluated for 4 weeks. T regulatory cells were identified by labeling with anti-CD4 and anti-Foxp3. Th17 cells were identified by labeling with anti-CD4 and anti-IL-17. Data are presented as mean ± standard error of the mean, where, *P < 0.05 versus N. N = 9, NM = 8, H = 8, HM = 9, NE = 8, NEM = 8, HE = 8, HEM = 8. Normolipidic control (N); Normolipidic + melanoma (NM); High-fat control (H); High-fat + melanoma (HM); Normolipidic control + physical exercise (NE); Normolipidic melanoma + physical exercise (NEM); High-fat control + physical exercise (HE); High-fat melanoma + physical exercise (HEM)