Fig. 4

Effect of a high-fat diet and moderate physical exercise on lymphocyte proliferation in tumor-bearing mice. C57BL6 mice were maintained on a diet containing either 60% or 10% of energy from lipids and were either trained on a treadmill at moderate intensity for 8 weeks or left untrained. Subsequently, B16F10 tumor cells were injected, and animals were evaluated for 4 weeks for lymphocyte proliferation as described in the Methods. Data are presented as mean ± standard error of the mean. *P < 0.05 versus N sedentary; ^&P < 0.05 versus HM sedentary; ^aP < 0.05 versus NM sedentary; ^b P < 0.05 versus H exercise; ^cP < 0.05 versus N exercise. N = 9, NM = 8, H = 8, HM = 9, NE = 8, NEM = 8, HE = 8, HEM = 8. Normolipidic control (N); Normolipidic + melanoma (NM); High-fat control (H); High-fat + melanoma (HM); Normolipidic control + physical exercise (NE); Normolipidic melanoma + physical exercise (NEM); High-fat control + physical exercise (HE); High-fat melanoma + physical exercise (HEM)