Fig. 6

Cytokine levels in the supernatant from cultures of lymph nodes lymphocytes isolated from mice with and without melanoma, either with or without a high-fat diet and moderate physical exercise. C57BL6 mice were maintained on a diet containing 60% or 10% of energy from lipids and were either trained on a treadmill at moderate intensity for 8 weeks or left untrained. Subsequently, B16F10 tumor cells were injected, and cytokine secretion in extracted lymphocytes was measure as described in the Methods. Data are presented as mean ± standard error of the mean, where, ^&P < 0.05 vs. sedentary high-fat diet melanoma. N = 9, NM = 8, H = 8, HM = 9, NE = 8, NEM = 8, HE = 8, HEM = 8