Fig. 6

The unique transcriptome alteration of PKAi and control mice induced by HFD. Mice were fed with HFD or CD from 8-week old for 2 months. Liver tissues were harvested to perform the RNA sequencing. a-b The heatmap (a) and top 10 enriched GO terms (b) of DEGs in the liver tissue of control mice vs. PKAi mice with HFD feeding. c Venn diagram showing the co-altered or differentially-altered DEGs in PKAi and control mice: orange and blue areas represent co-altered genes, either up- (orange) or down-regulated (blue) on the stimulation of HFD; red and purple areas represent differentially-altered genes in control mice, either up- (red) or down-regulated (purple) on the stimulation of HFD; yellow and green areas represent differentially-altered genes in PKAi mice, either up- (yellow) or down-regulated (green) after the stimulation of HFD. d-e Enriched pathways of differentially-altered genes in PKAi mice (d) and control mice (e). Each node represents a pathway, and the nodes in one color represent a cluster of pathways. The nodes in different-colored circles represent the enriched pathways of DEGs of the same color displayed in c. The thickness of lines between nodes represents the kappa score of overlapping genes of the linking nodes, calculated by Metascape online tool. (N = 3)