The effect of western diet on mice brain lipid composition

Pakiet, Alicja; Jakubiak, Agnieszka; Czumaj, Aleksandra; Sledzinski, Tomasz; Mika, Adriana

doi:10.1186/s12986-019-0401-4

Nutrition & Metabolism

Table 3 Fatty acid content [%] in various lipid fractions in mice brain

From: The effect of western diet on mice brain lipid composition

	Procedure I (Kaluzny et al. [27])						Procedure II (Bodennec et al. [28])
	Acylglycerols		Polar lipids		Free fatty acids		Ceramides		Glycosphingolipids		Sphingomyelin
	SD	HFD	SD	HFD	SD	HFD	SD	HFD	SD	HFD	SD	HFD
SFA	47.21 ± 5.59	45.13 ± 4.99	48.88 ± 1.00	48.84 ± 0.96	84.36 ± 1.46	83.68 ± 1.98	63.19 ± 3.16	57.55 ± 4.87	58.47 ± 1.68	56.96 ± 0.85	43.15 ± 2.55	44.52 ± 2.44
MUFA	33.73 ± 1.38	36.39 ± 1.61^*	32.06 ± 1.89	29.99 ± 2.20	9.83 ± 1.39	9.79 ± 1.27	27.42 ± 2.06	30.32 ± 2.11	32.10 ± 1.57	31.85 ± 1.26	25.19 ± 2.43	23.22 ± 2.92
16:2 n-6	0.03 ± 0.02	0.09 ± 0.08	0.00 ± 0.01	0.00 ± 0.00	0.0005 ± 0.0005	0.002 ± 0.002	0.04 ± 0.01	0.02 ± 0.02	0.01 ± 0.00	0.00 ± 0.00	0.01 ± 0.00	0.01 ± 0.00
LA	14.96 ± 5.40	14.13 ± 5.68	0.26 ± 0.02	0.46 ± 0.04^#	2.38 ± 0.13	1.94 ± 0.55	0.53 ± 1.34	0.84 ± 0.44	0.28 ± 0.02	0.59 ± 0.12^*	0.23 ± 0.02	0.40 ± 0.09^*
20:2 n-6	0.10 ± 0.04	0.08 ± 0.03	0.08 ± 0.00	0.16 ± 0.03^#	0.12 ± 0.08	0.02 ± 0.02	0.09 ± 0.02	0.09 ± 0.04	0.07 ± 0.03	0.15 ± 0.01^*	0.07 ± 0.02	0.10 ± 0.03
DGLA	0.13 ± 0.03	0.10 ± 0.05	0.30 ± 0.03	0.34 ± 0.04	0.02 ± 0.01	0.08 ± 0.04	0.19 ± 0.05	0.25 ± 0.07	0.19 ± 0.02	0.24 ± 0.03^*	0.34 ± 0.02	0.36 0.03
ARA	2.70 ± 0.99	2.72 ± 0.56	6.95 ± 0.86	7.44 ± 0.84	2.69 ± 0.27	3.36 ± 0.02	4.64 ± 1.08	5.54 ± 1.20	4.19 ± 0.53	4.90 ± 0.80	9.79 ± 0.79	9.66 ± 0.61
AdA	0.15 ± 0.05	0.19 ± 0.05	2.18 ± 0.32	2.21 ± 0.19	0.09 ± 0.00	0.16 ± 0.00	0.34 ± 0.07	0.73 ± 0.58	0.55 ± 0.06	0.58 ± 0.07	4.17 ± 0.41	3.86 ± 0.46
PUFA n-6	18.10 ± 5.03	17.37 ± 5.14	10.12 ± 1.31	10.73 ± 0.96	5.32 ± 0.03	5.53 ± 0.53	7.09 ± 1.12	8.04 ± 2.08	5.43 ± 0.55	6.50 ± 0.91	15.17 ± 1.21	14.57 ± 0.85
ALA	0.07 ± 0.06	0.04 ± 0.01	0.02 ± 0.01	0.02 ± 0.01	0.01 ± 0.00	0.02 ± 0.02	0.06 ± 0.04	0.03 ± 0.01	0.02 ± 0.02	0.02 ± 0.02	0.01 ± 0.00	0.02 ± 0.00^*
EPA	0.18 ± 0.09	0.15 ± 0.08	0.31 ± 0.03	0.13 ± 0.01^#	0.11 ± 0.02	0.29 ± 0.03^*	0.34 ± 0.08	0.18 ± 0.12^*	0.13 ± 0.01	0.06 ± 0.02^#	0.46 ± 0.03	0.22 ± 0.05^#
DPA	0.07 ± 0.02	0.06 ± 0.03	0.34 ± 0.05	0.35 ± 0.05	0.03 ± 0.03	0.03 ± 0.00	0.09 ± 0.06	0.11 ± 0.03	0.10 ± 0.02	0.11 ± 0.03	0.54 ± 0.05	0.48 ± 0.07
DHA	0.52 ± 0.17	0.68 ± 0.06	7.99 ± 0.61	9.77 ± 0.66^*	0.37 ± 0.06	0.60 ± 0.11	1.58 ± 0.29	3.58 ± 1.50^*	3.63 ± 0.42	4.41 ± 0.15^*	15.38 ± 1.17	16.86 ± 0.92
PUFA n-3	0.85 ± 0.25	0.93 ± 0.14	8.65 ± 0.66	10.27 ± 0.68^#	0.51 ± 0.10	0.93 ± 0.09	2.08 ± 0.35	3.91 ± 1.54^*	3.88 ± 0.44	4.61 ± 0.14^*	16.39 ± 1.19	17.58 ± 0.97

p-value from t-test: * < 0.05, # < 0.001, AdA – adrenic acid (22:4 n-6), ALA – α-linolenic acid (18:3 n-3), ARA – arachidonic acid (20:4 n-6), DGLA –dihomo-γ-linolenic acid (20:3 n-6), DHA – docosahexaenoic acid (22:6 n-3), DPA – docosapentaenoic acid (22:5 n-3), EPA – eicosapentaenoic acid (20:5 n-3), ETA – eicosatetraenoic acid (20:5 n-3), LA – linoleic acid (18:2 n-6), MUFA – monounsaturated fatty acids, PUFA – polyunsaturated fatty acids, SFA – saturated fatty acids
Boldface, major groups of fatty acid

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