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Table 2 List of the primers used for qRT-PCR to analyze the expressions of genes involved in glucose metabolism

From: Higher glycolytic capacities in muscle of carnivorous rainbow trout juveniles after high dietary carbohydrate stimulus at first feeding

Genes Forward primers Reverse primers
luciferase 5′-CATTCTTCGCCAAAAGCACTCTG-3′ 5-AGCCCATATCCTTGTCGTATCCC-3′
ef1α 5′-TCCTCTTGGTCGTTTCGCTG-3′ 5-ACCCGAGGGACATCCTGTG-3′
glut2a 5′-GACAGGCACTCTAACCCTAG-3′ 5′CTTCCTGCGTCTCTGTACTG-3′
glut2b 5′-CTATCAGAGAACGGTACAGGG-3′ 5′CAGGAAGGATGACACCACG-3′
glut4a 5′-CATCTTTGCAGTGCTCCTTG-3′ 5′CAGCTCTGTACTCTGCTTGC-3′
glut4b 5′-TCGGCTTTGGCTTCCAATATG-3′ 5′GTTTGCTGAAGGTGTTGGAG-3′
hk1 5′-CTGGGACGCTGAAGACCAGA-3′ 5′-CGGTGCTGCATACCTCCTTG-3′
hk2 5′-GGGACACCGAGAACAAGGG-3′ 5′-TCCCTTTGTCATCCTGTGCT-3′
pkmaa 5′-ACATTGCCCCCTACAGTTAC-3′ 5′-AAGTGGAAATGAATGGGACGT-3′
pkmab 5′-TGCTGAGGGCAGTGACGTA-3′ 5′-AGCTCCTCAAACAGCTGTCTG-3′
pkmba 5′-CAAGCCTGCCAACGATGTC-3′ 5′-CAAGGAACAAGCACAACACG-3′
pkmbb 5′-CAACTGTGACGAGAAGCACC-3′ 5′-GAGCCCAGAGTACCACCATT-3′
gcka 5′-CTGCCCACCTACGTCTGT-3′ 5′-GTCATGGCGTCCTCAGAGAT-3′
gckb 5′-TCTGTGCTAGAGACAGCCC-3′ 5′-CATTTTGACGCTGGACTCCT-3′
pfkla 5′-GATCCCTGCCACCATCAGTA-3′ 5′-GTAACCACAGTAGCCTCCCA-3′
pfklb 5′-AGTGCTCGCTGTAAGGTCTT-3′ 5′-GTGATCCGGCCTTTCTGAAC-3′
pklr 5′-CCATCGTCGCGGTAACAAGA-3′ 5′-GCCCCTGGCCTTTCCTATGT-3′
pfkmaa 5′-GTCAGTCTGTCCGGTAACCA-3′ 5′-ATCTGGAGGGTTGATGTGGG-3′
pfkmab 5′-TCAGCGGAGGAGGCTAATC-3′ 5′-GACTCTGTGCAGTAGTCGTG-3′
pfkmba 5′-CTGGGCATGAAAAGGCGAT-3′ 5′-GTCTTCTTGATGATGTGCTCCA-3′
pfkmbb 5′-CGGTCGTATCTTTGCCAACATG-3′ 5′-TGTCCATTTCCACAGTGTCATATT-3′
pck1 5′-ACAGGGTGAGGCAGATGTAGG −3′ 5′-CTAGTCTGTGGAGGTCTAAGGGC −3′
pck2 5′-ACAATGAGATGATGTGACTGCA-3′ 5′-TGCTCCATCACCTACAACCT-3′
fbp1b1 5′-CTCTCAAGAACCTCTACAGCCT-3′ 5′-TCAGTTCTCCCGTTCCCTTC-3′
fbp1b2 5′-ATCAGCAGGAATAGGTCGCG-3′ 5′-CCTCCTCCAGCACGAATCTC-3′
fbp1a 5′-GACAGAGGACGACCCGTG-3′ 5′-GTACTGACCGGGTCCAACAT −3′
g6pca 5′- GATGGCTTGACGTTCTCCT-3′ 5′- AGATCCAGGAGAGTCCTCC-3′
g6pcb1 5′-AGGGACAGTTCGAAAATGGAG-3′ 5′-CCAGAGAGGGAAGAAGATGAAGA-3′
g6pcb2 5′-CCTGCGGAACACCTTCTTTG-3′ 5′-TCAATTTGTGGCGCTGATGAG-3′
ldhaa 5′-GTGTTTCTCAGCGTTCCCTG-3′ 5′-GTTACAGAAGGGCACACAG-3′
ldhab 5′-GTGTTCCTCAGTGTGCCATG-3′ 5′-TTGCTGATAAATTAACCCTCCG-3′
slc16a3a 5′-TAGTGATGTCAAGGCACCAGAT-3′ 5′-CACTCCGAACTCCCTGATCAAC-3′
slc16a3b 5′-GAGTTGCAGGCTGTAGACC-3′ 5′-GCTCACCACAAACACAGGG-3′
  1. Ef1α: elongation factor 1 alpha; glut, glucose transporter; hk, hexokinase; pkm, pyruvate kinase (muscle); gck, glucokinase; pfkl,phosphofructokinase (liver); pklr, pyruvate kinase (liver and red blood cell); pfkm, phosphofructokinase (muscle); pck, phosphoenol pyruvate carboxykinases (cytosolic pck1 and mitochondrial pck2); fbp, fructose 1, 6-bisphosphatase; g6pc, glucose 6-phosphatase; ldha,Lactate dehydrogenase A; Slc16a3, solute carrier family 16 (monocarboxylic acid transporters) member 3