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Table 2 List of the primers used for qRT-PCR to analyze the expressions of genes involved in glucose metabolism

From: Higher glycolytic capacities in muscle of carnivorous rainbow trout juveniles after high dietary carbohydrate stimulus at first feeding

Genes

Forward primers

Reverse primers

luciferase

5′-CATTCTTCGCCAAAAGCACTCTG-3′

5-AGCCCATATCCTTGTCGTATCCC-3′

ef1α

5′-TCCTCTTGGTCGTTTCGCTG-3′

5-ACCCGAGGGACATCCTGTG-3′

glut2a

5′-GACAGGCACTCTAACCCTAG-3′

5′CTTCCTGCGTCTCTGTACTG-3′

glut2b

5′-CTATCAGAGAACGGTACAGGG-3′

5′CAGGAAGGATGACACCACG-3′

glut4a

5′-CATCTTTGCAGTGCTCCTTG-3′

5′CAGCTCTGTACTCTGCTTGC-3′

glut4b

5′-TCGGCTTTGGCTTCCAATATG-3′

5′GTTTGCTGAAGGTGTTGGAG-3′

hk1

5′-CTGGGACGCTGAAGACCAGA-3′

5′-CGGTGCTGCATACCTCCTTG-3′

hk2

5′-GGGACACCGAGAACAAGGG-3′

5′-TCCCTTTGTCATCCTGTGCT-3′

pkmaa

5′-ACATTGCCCCCTACAGTTAC-3′

5′-AAGTGGAAATGAATGGGACGT-3′

pkmab

5′-TGCTGAGGGCAGTGACGTA-3′

5′-AGCTCCTCAAACAGCTGTCTG-3′

pkmba

5′-CAAGCCTGCCAACGATGTC-3′

5′-CAAGGAACAAGCACAACACG-3′

pkmbb

5′-CAACTGTGACGAGAAGCACC-3′

5′-GAGCCCAGAGTACCACCATT-3′

gcka

5′-CTGCCCACCTACGTCTGT-3′

5′-GTCATGGCGTCCTCAGAGAT-3′

gckb

5′-TCTGTGCTAGAGACAGCCC-3′

5′-CATTTTGACGCTGGACTCCT-3′

pfkla

5′-GATCCCTGCCACCATCAGTA-3′

5′-GTAACCACAGTAGCCTCCCA-3′

pfklb

5′-AGTGCTCGCTGTAAGGTCTT-3′

5′-GTGATCCGGCCTTTCTGAAC-3′

pklr

5′-CCATCGTCGCGGTAACAAGA-3′

5′-GCCCCTGGCCTTTCCTATGT-3′

pfkmaa

5′-GTCAGTCTGTCCGGTAACCA-3′

5′-ATCTGGAGGGTTGATGTGGG-3′

pfkmab

5′-TCAGCGGAGGAGGCTAATC-3′

5′-GACTCTGTGCAGTAGTCGTG-3′

pfkmba

5′-CTGGGCATGAAAAGGCGAT-3′

5′-GTCTTCTTGATGATGTGCTCCA-3′

pfkmbb

5′-CGGTCGTATCTTTGCCAACATG-3′

5′-TGTCCATTTCCACAGTGTCATATT-3′

pck1

5′-ACAGGGTGAGGCAGATGTAGG −3′

5′-CTAGTCTGTGGAGGTCTAAGGGC −3′

pck2

5′-ACAATGAGATGATGTGACTGCA-3′

5′-TGCTCCATCACCTACAACCT-3′

fbp1b1

5′-CTCTCAAGAACCTCTACAGCCT-3′

5′-TCAGTTCTCCCGTTCCCTTC-3′

fbp1b2

5′-ATCAGCAGGAATAGGTCGCG-3′

5′-CCTCCTCCAGCACGAATCTC-3′

fbp1a

5′-GACAGAGGACGACCCGTG-3′

5′-GTACTGACCGGGTCCAACAT −3′

g6pca

5′- GATGGCTTGACGTTCTCCT-3′

5′- AGATCCAGGAGAGTCCTCC-3′

g6pcb1

5′-AGGGACAGTTCGAAAATGGAG-3′

5′-CCAGAGAGGGAAGAAGATGAAGA-3′

g6pcb2

5′-CCTGCGGAACACCTTCTTTG-3′

5′-TCAATTTGTGGCGCTGATGAG-3′

ldhaa

5′-GTGTTTCTCAGCGTTCCCTG-3′

5′-GTTACAGAAGGGCACACAG-3′

ldhab

5′-GTGTTCCTCAGTGTGCCATG-3′

5′-TTGCTGATAAATTAACCCTCCG-3′

slc16a3a

5′-TAGTGATGTCAAGGCACCAGAT-3′

5′-CACTCCGAACTCCCTGATCAAC-3′

slc16a3b

5′-GAGTTGCAGGCTGTAGACC-3′

5′-GCTCACCACAAACACAGGG-3′

  1. Ef1α: elongation factor 1 alpha; glut, glucose transporter; hk, hexokinase; pkm, pyruvate kinase (muscle); gck, glucokinase; pfkl,phosphofructokinase (liver); pklr, pyruvate kinase (liver and red blood cell); pfkm, phosphofructokinase (muscle); pck, phosphoenol pyruvate carboxykinases (cytosolic pck1 and mitochondrial pck2); fbp, fructose 1, 6-bisphosphatase; g6pc, glucose 6-phosphatase; ldha,Lactate dehydrogenase A; Slc16a3, solute carrier family 16 (monocarboxylic acid transporters) member 3