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Table 2 List of the primers used for qRT-PCR to analyze the expressions of genes involved in glucose metabolism

From: Higher glycolytic capacities in muscle of carnivorous rainbow trout juveniles after high dietary carbohydrate stimulus at first feeding

GenesForward primersReverse primers
luciferase5′-CATTCTTCGCCAAAAGCACTCTG-3′5-AGCCCATATCCTTGTCGTATCCC-3′
ef1α5′-TCCTCTTGGTCGTTTCGCTG-3′5-ACCCGAGGGACATCCTGTG-3′
glut2a5′-GACAGGCACTCTAACCCTAG-3′5′CTTCCTGCGTCTCTGTACTG-3′
glut2b5′-CTATCAGAGAACGGTACAGGG-3′5′CAGGAAGGATGACACCACG-3′
glut4a5′-CATCTTTGCAGTGCTCCTTG-3′5′CAGCTCTGTACTCTGCTTGC-3′
glut4b5′-TCGGCTTTGGCTTCCAATATG-3′5′GTTTGCTGAAGGTGTTGGAG-3′
hk15′-CTGGGACGCTGAAGACCAGA-3′5′-CGGTGCTGCATACCTCCTTG-3′
hk25′-GGGACACCGAGAACAAGGG-3′5′-TCCCTTTGTCATCCTGTGCT-3′
pkmaa5′-ACATTGCCCCCTACAGTTAC-3′5′-AAGTGGAAATGAATGGGACGT-3′
pkmab5′-TGCTGAGGGCAGTGACGTA-3′5′-AGCTCCTCAAACAGCTGTCTG-3′
pkmba5′-CAAGCCTGCCAACGATGTC-3′5′-CAAGGAACAAGCACAACACG-3′
pkmbb5′-CAACTGTGACGAGAAGCACC-3′5′-GAGCCCAGAGTACCACCATT-3′
gcka5′-CTGCCCACCTACGTCTGT-3′5′-GTCATGGCGTCCTCAGAGAT-3′
gckb5′-TCTGTGCTAGAGACAGCCC-3′5′-CATTTTGACGCTGGACTCCT-3′
pfkla5′-GATCCCTGCCACCATCAGTA-3′5′-GTAACCACAGTAGCCTCCCA-3′
pfklb5′-AGTGCTCGCTGTAAGGTCTT-3′5′-GTGATCCGGCCTTTCTGAAC-3′
pklr5′-CCATCGTCGCGGTAACAAGA-3′5′-GCCCCTGGCCTTTCCTATGT-3′
pfkmaa5′-GTCAGTCTGTCCGGTAACCA-3′5′-ATCTGGAGGGTTGATGTGGG-3′
pfkmab5′-TCAGCGGAGGAGGCTAATC-3′5′-GACTCTGTGCAGTAGTCGTG-3′
pfkmba5′-CTGGGCATGAAAAGGCGAT-3′5′-GTCTTCTTGATGATGTGCTCCA-3′
pfkmbb5′-CGGTCGTATCTTTGCCAACATG-3′5′-TGTCCATTTCCACAGTGTCATATT-3′
pck15′-ACAGGGTGAGGCAGATGTAGG −3′5′-CTAGTCTGTGGAGGTCTAAGGGC −3′
pck25′-ACAATGAGATGATGTGACTGCA-3′5′-TGCTCCATCACCTACAACCT-3′
fbp1b15′-CTCTCAAGAACCTCTACAGCCT-3′5′-TCAGTTCTCCCGTTCCCTTC-3′
fbp1b25′-ATCAGCAGGAATAGGTCGCG-3′5′-CCTCCTCCAGCACGAATCTC-3′
fbp1a5′-GACAGAGGACGACCCGTG-3′5′-GTACTGACCGGGTCCAACAT −3′
g6pca5′- GATGGCTTGACGTTCTCCT-3′5′- AGATCCAGGAGAGTCCTCC-3′
g6pcb15′-AGGGACAGTTCGAAAATGGAG-3′5′-CCAGAGAGGGAAGAAGATGAAGA-3′
g6pcb25′-CCTGCGGAACACCTTCTTTG-3′5′-TCAATTTGTGGCGCTGATGAG-3′
ldhaa5′-GTGTTTCTCAGCGTTCCCTG-3′5′-GTTACAGAAGGGCACACAG-3′
ldhab5′-GTGTTCCTCAGTGTGCCATG-3′5′-TTGCTGATAAATTAACCCTCCG-3′
slc16a3a5′-TAGTGATGTCAAGGCACCAGAT-3′5′-CACTCCGAACTCCCTGATCAAC-3′
slc16a3b5′-GAGTTGCAGGCTGTAGACC-3′5′-GCTCACCACAAACACAGGG-3′
  1. Ef1α: elongation factor 1 alpha; glut, glucose transporter; hk, hexokinase; pkm, pyruvate kinase (muscle); gck, glucokinase; pfkl,phosphofructokinase (liver); pklr, pyruvate kinase (liver and red blood cell); pfkm, phosphofructokinase (muscle); pck, phosphoenol pyruvate carboxykinases (cytosolic pck1 and mitochondrial pck2); fbp, fructose 1, 6-bisphosphatase; g6pc, glucose 6-phosphatase; ldha,Lactate dehydrogenase A; Slc16a3, solute carrier family 16 (monocarboxylic acid transporters) member 3