Excessive BCAA regulates fat metabolism partially through the modification of m6A RNA methylation in weanling piglets

Table 1 Composition of the experimental diets (As-Fed basis)

Items	Treatments^a
Items	L-BCAA	N-BCAA	H-BCAA
Ingredient (%)
Corn (7.8%, CP)	69.24	69.38	69.50
Soybean meal (47%, CP)	9.45	9.15	8.55
Concentrated soybean protein (65.2%, CP)	5.00	5.00	5.00
Fish meal (62.5%, CP)	4.00	4.00	4.00
Whey powder (3.8%, CP)	5.00	5.00	5.00
Soybean oil powder	1.50	1.50	1.50
L-lysine HCl	0.66	0.66	0.67
_D,L-methionine	0.30	0.30	0.31
L-threonine	0.30	0.30	0.32
L-tryptophan	0.08	0.08	0.09
L-leucine	0	0.09	0.82
L-isoleucine	0	0.13	0.51
L-valine	0	0.23	0.70
L-alanine	1.44	1.15	0
Salt	0.30	0.30	0.30
Calcium formate	0.60	0.60	0.60
Dicalcium phosphate	1.13	1.13	1.13
Premix^b	1.00	1.00	1.00
Calculated Nutrient Content (%)
Crude protein	17.45	17.45	17.45
Digestive energy (MJ/kg)	14.68	14.70	14.64
Total Ca	0.80	0.80	0.80
Total P	0.66	0.66	0.66
Available P	0.44	0.44	0.44
Lys	1.36	1.35	1.35
Met + Cys	0.75	0.75	0.74
Threonine	0.79	0.79	0.79
Tryptophan	0.22	0.22	0.22
Leucine	1.25	1.35	2.03
Isoleucine	0.55	0.69	1.04
Valine	0.62	0.86	1.29

^a L-BCAA Low dose BCAA diet, N-BCAA Normal dose BCAA diet, H-BCAA High dose BCAA diet. ^b Provided per kilogram of diet (as-fed basis): VA, 13000 IU; VD₃, 40 00 IU; VE, 39.4 mg; VB₁, 6.2 mg; VB₂, 11.2 mg; VB₆, 12.2 mg; VB₁₂, 4 mg; VK, 4.0 mg; niacin, 45.0 mg; folate, 2.2 mg; pantothenic acid, 25.0 mg; biotin, 0.2 mg; choline chloride, 500.0 mg; Cu, 35.0 mg; Fe, 105.0 mg; Mn, 25.0 mg; Zn, 1600.0 mg; I, 0.3 mg; Se, 0.6 mg; Co, 0.3 mg

ISSN: 1743-7075