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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Indirubin, a small molecular deriving from connectivity map (CMAP) screening, ameliorates obesity-induced metabolic dysfunction by enhancing brown adipose thermogenesis and white adipose browning

Fig. 1

CMAP based screen identifies indirubin as a regulator of UCP1. a Flow chart depicting the process of CMAP screen using CITGeneDB to identify potential small-molecule for inducing UCP1. b, c The chemical structure and the CAS number of indirubin. d Dose-dependent effect of indirubin on Ucp1 expression in differentiated C3H10T1/2 cells on day 6 of brown adipogenesis. e RT-qPCR analysis of genes related to thermogenesis, fatty acid oxidation, mitochondrial biogenesis and common adipogenic marks in differentiated C3H10T1/2 cells (6 days of differentiation) after indirubin (10 μM) treatment. f Oxygen consumption rates (OCR) was measured in differentiated C3H10T1/2 cells (6 days of differentiation) in the presence or absence of indirubin using a Seahorse XF24 analyzer. g Basal oxygen consumption and maximum respiratory capacity from seahorse assay were determined. Data in D-G are presented as mean ± SD of six independent experiments performed in duplicate. *p < 0.05, **p < 0.01 compared with vehicle

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Nutrition & Metabolism

ISSN: 1743-7075

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