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Fig. 6 | Nutrition & Metabolism

Fig. 6

From: Indirubin, a small molecular deriving from connectivity map (CMAP) screening, ameliorates obesity-induced metabolic dysfunction by enhancing brown adipose thermogenesis and white adipose browning

Fig. 6

Indirubin increases BAT activity by promoting thermogenesis and mitochondrial biogenesis in HFD-induced obese mice. a-e RT-qPCR analysis of genes related to thermogenesis a, fatty acid oxidation b, lipolysis c, common adipogenic marks d and mitochondrial biogenesis in BAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. f Measurement of mtDMA copy number in BAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. g Immunohistochemistry (IHC) staining with a UCP1-specific antibody in BAT of mice fed NCD or HFD after 8-week treatment with either vehicle or indirubin. Scale bar = 50 μm. h-i Western blot analysis of proteins levels of UCP1, PGC1α, VDAC1, and OXPHOS in BAT of mice fed NCD h or HFD i after 8-week treatment with either vehicle or indirubin. β-tubulin serves as a loading control. Data in A-F are presented as mean ± SD (n = 6 in each group). *p < 0.05, **p < 0.01 compared with HFD + vehicle group

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Nutrition & Metabolism

ISSN: 1743-7075

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