Fig. 4

Flutamide reversed the training-induced increases of AR in the muscles of rats. The elevated protein levels of AR in the gastrocnemius through resistance training (a) and in the soleus through endurance training (b) were both reversed by flutamide treatment. Protein levels of AR and GAPDH in gastrocnemius and soleus were determined by Western blot, and levels of AR were quantified and normalized against GAPDH. The AR/GAPDH ratio of control group was identified as 1, and the relative values of AR in experimental groups are expressed as fold of control. C: control; F: flutamide embedded; R: resistance training; R + F: resistance training plus flutamide embedded; E: endurance training; E + F: endurance training plus flutamide embedded. Data are expressed as the mean ± SD of at least three independent experiments. ^*P<0.05 vs C; ^#P<0.05, ^##P<0.01 vs the relative training groups