Fig. 6

Flutamide down-regulated the protein levels of IGF-1, IGF-1R and mTOR in the muscles of training rats. a: in gastrocnemius, resistance training-induced elevations in the protein levels of IGF-1, IGF-1R and mTOR were reversed by flutamide treatment; b: in soleus, endurance training raised the protein levels of IGF-1 and IGF-1R rather than mTOR, but all of them were decreased by flutamide treatment. Protein levels of IGF-1, IGF-1R, mTOR and GAPDH in gastrocnemius and soleus were determined by Western blot, and levels of IGF-1, IGF-1R, and mTOR were quantified and normalized against GAPDH. The ratio of IGF-1/GAPDH, IGF-1R/GAPDH, or mTOR/GAPDH in control group was identified as 1, and the relative values of the above indicators in experimental groups are expressed as fold of control. C: control; F: flutamide embedded; R: resistance training; R + F: resistance training plus flutamide embedded; E: endurance training; E + F: endurance training plus flutamide embedded. Data are expressed as the mean ± SD of at least three independent experiments. ^*P<0.05, ^**P<0.01 vs C; ^#P<0.05, ^##P<0.01 vs the relative training groups