Fig. 7

Trainings induced the enhancements of PI3K and Akt in activities rather than protein levels in the muscles of rats, which were reversed by flutamide. Elevations of p-PI3K/PI3K ratio and p-Akt/Akt ratio in the gastrocnemius through resistance training (a) and in the soleus through endurance training (b) were reversed by flutamide treatment. No change was observed in the ratios of PI3K/GAPDH and Akt/GAPDH among groups (so the graphs do not show). Protein levels of p-PI3K, PI3K, p-Akt, Akt and GAPDH in gastrocnemius and soleus were determined by Western blot, and protein levels and activity of PI3K were reflected by PI3K/GAPDH ratio and p-PI3K/PI3K ratio, respectively. Akt’s protein level and activity were described similarly. The p-PI3K/PI3K ratio or p-Akt/Akt ratio of control group was identified as 1, and the relative values of p-PI3K/PI3K or p-Akt/Akt in experimental groups are expressed as the fold of control. C: control; F: flutamide embedded; R: resistance training; R + F: resistance training plus flutamide embedded; E: endurance training; E + F: endurance training plus flutamide embedded. Data are expressed as the mean ± SD of at least three independent experiments. ^*P<0.05, ^**P<0.01 vs C; ^#P<0.05, ^##P<0.01 vs the relative training groups