Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis

Table 2 Primer sequences used for real-time polymerase chain reaction

Gene	Gene ID	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)
FXR	20186	CAGATTTCCTCCTCGTCTTAC	CCTGAGTTCATAGATGCCA
SHP	23957	TTCCTTGCTTTGGATACAGTG	GAGGTTTGGGGAGTCATCA
SREBP1	20787	GGCTTGGTGATGCTATGTTGA	GGCAAAGGAACAACTGAGACC
FASN	14104	GTGTGGTGGGTT TGG TGA AT	AGATGTGTTGCT GAG GTT GGA
SCD1	20249	TGAGGG GCA GTG TCT GTA A	GGAAACTAAAGCAATGGA TGG
PPARα	19013	GAGGATGGGGACTTTTGTTCT	GGC TTT TTG GCT GTA GGA GG
CPT1	12894	ACC CAG TCA GAT TCC AAC C	ACAAAGCACCCA TTA CTT GAG
MTTP	17777	CCACCAGAATCGTAAGGTTCA	GGACAGCAGGAT GTT CTT CAC
CYP7A1	13122	GTACCTTGATGAAAGTGGGAA	ATTGCT TGA TTT CTT GGA CAG
FGFR4	14186	CCT TCT GTT CCA GCC TTA TG	TTC TCT GAG GAT GAG TCC AAG
BSEP	27413	AAT GTT CAG TTC CTC CGT TC	GTC CCC ATA CTT GAT GTT GTC
NTCP	20493	GTCATCAATGTGGGCAACAGC A	TGAAAGGCATCAGGGAGGAGG TA
KLB	83379	CCT TCT GTT CCA GCC TTA TG	TTC TCT GAG GAT GAG TCC AAG
FGF15	14170	GAAGCCAGA AGG TAT GAA GTC	CCA AGT TTG TAA CCC CAG T
β-Actin	81822	CCT CTA TGC CAA CAC AGT	AGC CAC CAA TCC ACA CAG
LPL	16956	CGA GAGCGAGAA CAT TCC CT	TGT CCA CCT CCG TGT AAA TCA A

FXR farnesoid X receptor, SHP small heterodimer partner, SREBP1 sterol-regulatory element-binding protein 1, SCD1 stearyl coenzyme A desaturation enzyme 1, FASN fatty acid synthetase, PPARα peroxisome proliferator-activated receptor alpha, CPT1 carnitine palmitoyl transferase 1, MTTP microsomal triglyceride transfer protein, CYP7A1 cholesterol 7-alpha hydroxy-lase, FGFR4 fibroblast growth factor receptor 4, BSEP bile salt export protein, NTCP, Na+-taurocholate co-transporting polypeptide, KLB Klotho beta, FGF15 fibroblast growth factor 15, LPL lipoprotein lipase

ISSN: 1743-7075