Table 1 Primers for real time PCR assays
Gene | Forward primers | Reverse primers |
|---|---|---|
Cyp7a1 | GAGCCCTGAAGCAATGAAAG | GCTGTCCGGATATTCAAGGA |
Cyp8b1 | GGACAGCCTATCCTTGGTGA | GACGGAACTTCCTGAACAGC |
Cyp4a10 | AATGGGAACGGCTGGCTGA | TGGCCAAGCGGCCATTGGAA |
Cyp4a14 | GGCGAAAGAAAGTCAGGTT | AGGCAGTGTTCAGTTGGA |
Cyp4a31 | AATGGGAACGGCTGGCTGGT | TGGACAAGCGGCCATTGGA |
Cyp2c70 | TGGAGCCGGAACAGAGACAACGA | ACGGCATGTGGTTCCTGTCCTGCA |
Cyp3a59 | CAGGCTAGAAAGGGTCTCAAAA | AGTTTCATGCTGATGAGAGCAA |
Gsta3 | AGGAGTGGCGGATCTGGAGA | CGGGTCCAGCTCTTCCACAT |
Gstm6 | GCCATGGTTTGCAGGGGACA | TGGAAGGAAGCGGCTGGTCT |
Gstm7 | CCATGGTTCGCAGGGGACAA | TTGCTGCCCCAAGTTGCCA |
Nat8f1 | ACAGCATCGAGGACAGGGGA | GCCTGCCTTCTTGAAGCCCA |
Slc22a30 | GGAAGTATTATTTGTCTCCCATCG | AGGATCCTACTGCCATTATCAGAG |
Slc22a5 | ACGAAGCCTCAGTTGCACCA | ACAACAGCCAGGCCAGCACA |
Slc30a10 | GACTTTCATTTGCTGTGCTGAG | GTGACAGGACATAAGGCAATGA |
Slc4a4 | CTTACAGAATCCCCAAGACTGG | AAGTTGCATAGGAAAAGGGACA |
Slco1a4 | TACACGCTGGGTCGGTGCTT | TCGGATTGCAGGAGAGGCT |
Ugt2b35 | GCCCTTGCCCAGATTCCACA | AGAGTCCATTCGCGCCACCA |
Cyp3a11 | TGCCTTGGCATGAGGTTTGC | TGACTGCATCCCGTGGCACA |
Cyp2c70 | TGGAGCCGGAACAGAGACAACGA | ACGGCATGTGGTTCCTGTCCTGCA |
Gapdh | TGTGTCCGTCGTGGATCTGA | CCTGCTTCACCACCTTCTTGA |