Fig. 4

AE and metformin regulate miR-146a and miR-155 as well as their target genes in high fat treated aortas and HAECs. a, relative expression levels of miR-146a, miR-155 and miR-21 in rat aortas. b, relative mRNA levels of IRAK1, TRAF6 and NF-κB p65 in rat aortas. c, relative protein levels of IRAK1, TRAF6 and NF-κB p65 in rat aortas. d, relative expression levels of miR-146a, miR-155 and miR-21 in HAECs. e, relative mRNA levels of IRAK1, TRAF6 and NF-κB p65 in HAECs. f, relative protein levels of IRAK1, TRAF6 and NF-κB p65 in HAECs. Data are presented as mean ± SEM. ^*P < 0.05 versus NC or BSA group. ^**P < 0.01 versus NC or BSA group. ^#P<0.05 versus HF or PA group. ^##P<0.01 versus HF or PA group. n = 6–8 rats per group for animal study. n = 5 per group for HAECs study