Fig. 2

RES affected the frequency and activation of CD4⁺ T cells. Peripheral blood of mice was collected at the end of the 20th week and lymphocyte isolate medium was used to obtain peripheral blood mononuclear cells (PBMC). a-b CD4 FITC antibody was used and the frequency of CD4⁺ T cells in PBMC was detected by flow cytometry (n = 4). c-d CD4 FITC, CD25 APC, CD44 PerCP and CD62L PE antibody were used to stain the cells and the frequency of CD25⁺, CD44⁺ and CD62L⁺ cells in CD4⁺ T cells were analyzed by flow cytometry (n = 4). Data are represented with mean ± SD, #P < 0.05, ##P < 0.01, ###P < 0.001 versus the control group; *P < 0.05, **P < 0.01 versus the HFD + LPS group