Fig. 4

RES inhibited the expression of Dnmt1 and Dnmt3b in CD4⁺ T cells. CD4⁺ T cells were stimulated by 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28 and cultured in a cell incubator. 0.1 μg/mL LPS and 20, 40, 80 μM of RES were added and cells were collected 24 h later. a mRNA level of Dnmt in CD4⁺ T cells detected by qRT-PCR (n = 4). b-c The protein level of Dnmt in CD4⁺ T cells detected by WB (n = 4). 5-Aza were pretreated for 36 h, and cells were collected after the treatment of LPS and 40 μM of RES. d mRNA level of Dnmt in CD4⁺ T cells (n = 4). e-f The protein level of Dnmt in CD4⁺ T cells (n = 4). g-h The expression of proliferation marker, Ki67 was observed by flow cytometry (n = 4). i-j The expression of activation markers including CD25 and CD44 of CD4⁺ T cells were observed by flow cytometry (n = 4). Data are represented with mean ± SD, #P < 0.05, ##P < 0.01, ###P < 0.001 versus the control group; *P < 0.05, **P < 0.01, ***P < 0.001 versus the TCR + LPS group