Fig. 1

Changes in BW, energy intake and glucose metabolism in HFD- or NCD-fed adult and juvenile rats. a, b: Changes in BW a and energy intake b in the Adult group (n = 8, 8). c, d: Changes in BW c and energy intake d in the Juvenile group (n = 8, 8).e: Blood glucose levels after glucose tolerance test (GTT: 2 g/kg) in the Adult group (n = 4, 4). f: Plasma insulin levels after glucose injection (2 g/kg) at 0, 30, 60,120 min in Adult group (n = 4, 4). g: Blood glucose levels after insulin tolerance test (ITT: 1 IU/kg) in the Adult group. (n = 4, 4) H: Blood glucose levels after GTT (2 g/kg) in the Juvenile group (n = 4, 4). i: Plasma insulin levels after glucose injection (2 g / kg) at 0, 30, 60,120 min in the Juvenile group (n = 4, 3). j: Blood glucose levels after ITT (1 IU/kg) in the juvenile rats (n = 4, 4). GTT and ITT were performed at 69 and 72 days from onset of HFD feeding, respectively. Open circle and closed circle indicate normal chow diet (NCD) and high fat diet (HFD) feeding, respectively. a-d, e, g, h, j, * P < 0.05, ** P < 0.01. Two-way ANOVA, Tukey’s multiple range test. f, i, * P < 0.05, unpaired t-test