Fig. 6

The mRNA of PPARα was detected by Q-PCR (a,b), and the protein of PPARα was detected by western blot (g,h,i), in the in vivo and in vitro models of NAFLD. After transfection of siTET1, cells were treated with a PPARα agonist to measure intracellular TG content and β-HB content in the cell supernatant (C,D,E,F). After treatment with different concentrations of methylase inhibitor 5-AZA, mRNA of PPARα was detected by Q-PCR (j,k). ChIP experiments confirm that TET1 binds most strongly at PPARα promoters(− 415--115) in L02 and HepG2 cells (l,m), hMeDIP-Q-PCR test of the 5hmC enrichment of PPARα promoters(− 415--115) in L02 and HepG2 cells, after transfection of siTET1 (n), hMeDIP-Q-PCR test of the 5hmC enrichment of PPARα promoters(− 208--44) in mice after HFD feeding (o)