Fig. 4

Insulin signaling-related protein levels in rats fed diets low in protein or arginine. Six-week-old male Wistar rats were fed the 15PAA (n = 8), low Arg (n = 8), and 5PAA (n = 8) diets for 14 d. Whole-liver lysates were analyzed by immunoprecipitation and immunoblotting with antibodies against IRβ (b), IRS-2 (c), 4E-BP1 (d), phosphorylated 4E-BP1 (Thr37/46) (E), CD36 (f), and GAPDH as an internal control. Representative immunoblots (a) are shown. The values are the means ± standard errors. Asterisks indicate significant differences as assessed by one-way ANOVA with Bonferroni and Tukey–Kramer post-hoc tests (*p < 0.05; **p < 0.01)